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Registros recuperados: 6
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Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira Genet. Mol. Biol.
Caparroz,Renato; Mantellatto,Aline M.B.; Bertioli,David J.; Figueiredo,Marina G.; Duarte,José Maurício B..
The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer RNAs and the control region, as found in other deer. The genetic divergence between the mitogenome described here and a previously published report was ∼0.5%, with the control region and ND5 gene showing the highest intraspecific variation. Seven polymorphic loci were characterized using 15 unrelated individuals; there was moderate genetic variation across most loci (mean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Microsatellites; Mitogenome; Population genetics; 454-pyrosequencing.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300338
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Chromosomal similarity between the Scaly-headed parrot (Pionus maximiliani), the Short-tailed parrot (Graydidascalus brachyurus) and the Yellow-faced parrot (Salvatoria xanthops) (Psittaciformes: Aves): a cytotaxonomic analysis Genet. Mol. Biol.
Caparroz,Renato; Duarte,José Maurício Barbanti.
Behavior, morphology, allozyme studies and DNA hybridization and sequencing data all suggest the independent evolution of the Old and New World parrots and support tribe status for the American species, although the phylogenetic relationships within this tribe are still poorly understood. A previous study has shown that the Yellow-faced parrot (Amazona xanthops Spix 1824) exhibits large karyotypic differences compared to the other Amazona species and suggested that this species should be renamed Salvatoria xanthops, although the relationships between S. xanthops and the other New World parrots remain unclear. In the present work, we describe the karyotype of the Scaly-headed parrot (Pionus maximiliani, Kuhl 1820) and the karyotype and C-banding pattern of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome; Karyotype; Cytotaxonomy; Psittaciformes; Parrots.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400010
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Comparative analysis of microsatellite variability in five macaw species (Psittaciformes, Psittacidae): application for conservation Genet. Mol. Biol.
Presti,Flavia T.; Oliveira-Marques,Adriana R.; Caparroz,Renato; Biondo,Cibele; Miyaki,Cristina Y..
Cross-amplification was tested and variability in microsatellite primers (designed for Neotropical parrots) compared, in five macaw species, viz., three endangered blue macaws (Cyanopsitta spixii [extinct in the wild], Anodorhynchus leari [endangered] and Anodorhynchus hyacinthinus [vulnerable]), and two unthreatened red macaws (Ara chloropterus and Ara macao). Among the primers tested, 84.6% successfully amplified products in C. spixii, 83.3% in A. leari, 76.4% in A. hyacinthinus, 78.6% in A. chloropterus and 71.4% in A. macao. The mean expected heterozygosity estimated for each species, and based on loci analyzed in all the five, ranged from 0.33 (A. hyacinthinus) to 0.85 (A. macao). As expected, the results revealed lower levels of genetic variability...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Cross-amplification; Macaw; Microsatellite; Psittaciformes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200030
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Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci Genet. Mol. Biol.
Mantellatto,Aline Meira Bonfim; Caparroz,Renato; Christofoletti,Maurício Durante; Piovezan,Ubiratan; Duarte,José Maurício Barbanti.
Abstract The pampas deer (Ozotoceros bezoarticus) is close to being classified as ‘globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Fecal DNA; Microsatellites; Non-invasive methods; Pampas deer.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500774
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Genetic evaluation of the mating system in the blue-and-yellow macaw (Ara ararauna, Aves, Psittacidae) by DNA fingerprinting Genet. Mol. Biol.
Caparroz,Renato; Miyaki,Cristina Y.; Baker,Allan J..
More than 90% of birds are socially monogamous, although genetic studies indicate that many are often not sexually monogamous. In the present study, DNA fingerprinting was used to estimate the genetic relationships between nestlings belonging to the same broods to evaluate the mating system in the socially monogamous macaw, Ara ararauna. We found that in 10 of 11 broods investigated, the nestlings showed genetic similarity levels congruent with values expected among full-sibs, suggesting that they shared the same parents. However, in one brood, the low genetic similarity observed between nestlings could be a result of intraspecific brood parasitism, intraspecific nest competition or extra-pair paternity. These results, along with available behavioral and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ara ararauna; Breeding behavior; DNA fingerprinting; Monogamy; Psittacidae.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100027
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In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae) Genet. Mol. Biol.
Lima-Rezende,Cássia Alves; Fernandes,Gislaine Aparecida; Silva,Helder Elias da; Dobkowski-Marinho,Sarah; Santos,Victor Fernandes; Rodrigues,Fernando Pacheco; Caparroz,Renato.
Abstract The illegal trade is a major threat to many bird species, and parrots are common victims of this activity. Domestic and international pet markets are interested on different parrot species, such as the Blue-and-yellow Macaw (Ara ararauna). This South American macaw is not globally threatened, but is under protection from over-exploitation. This study aimed to identify and characterize novel microsatellite loci for population and parentage analysis of A. ararauna. Scaffold sequences of Ara macao available in the NCBI database were used for microsatellite searches using MsatCommander software. We tested a total of 28 loci, from which 25 were polymorphic, one was monomorphic, and two did not generated amplification products. For polymorphic loci, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Captive breeding; Illegal wildlife trade; Parentage; Psittacidae; Wildlife forensics.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100068
Registros recuperados: 6
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